Rabbit anti-GHRL [+Biotin]

Rabbit anti-GHRL Biotinylated Antibody, 50 ug

$220.00  
RB-08-0001B-50
+

Protein Information

Accession Number:
  • NM_016362
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (recommended work dilution= 1:10e4), Western Blotting (recommended work dilution= 1:1000-2000)
Conjugation/Tag:
Biotin
Host Species:
Rabbit
Preparation:
Immunogen was synthetic peptide derived from Human Ghrelin precursor. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Reconstitution:
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Clonality:
Polyclonal
Isotype:
IgG

Description

Ghrelin is synthesized as a preprohormone, and then proteolytically processed to yield a 28-amino acid peptide. It is a recently isolated brain-gut peptide that has growth hormone-releasing and appetiteinducing activities. Several recent studies have suggested that ghrelin plays a major role in the pathophysiology of drug-seeking behavior and anxiety. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor peptides that have a signal peptide followed by the ghrelin sequence. At the C-terminus of the ghrelin sequence, processing occurs at an uncommon Pro-Arg recognition site. In rat stomach, two isoforms of mRNA for pro-ghrelin are produced from the gene by an alternative splicing. One mRNA encodes the ghrelin precursor, and another encodes a des-Gln14- ghrelin precursor. O-octanoylation or O-decanoylation is essential for ghrelin activity. The Odecanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Specificity

The rabbit anti-human Ghrelin antibody detects specifically target derived from related-cell lysates protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.

References

  • Zhang J, Ren P, Avsian-Kretchmer O, Luo C, Rauch R, Klein C, Hsueh A (2005). "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake". Science 310 (5750): 996–9.
  • Garcia J, Garcia-Touza M, Hijazi R, Taffet G, Epner D, Mann D, Smith R, Cunningham G, Marcelli M (2005). "Active ghrelin levels and active to total ghrelin ratio in cancer-induced cachexia". J Clin Endocrinol Metab 90 (5): 2920–6.
Expiration:
12 months from the date of shipment when stored properly.

No posts found

Write A Review
U.S. Non-U.S.

Auto Western Service Available!

Thinking about using this antibody for western? Try our Auto Western service, which uses capillary immunoblotting to achieve ng to pg sensitivity. Just send us your samples and we will do the rest! Click here to learn more.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Auto-Western Blot Service