ELISA (recommended work dilution= 1:10e4), Western Blotting (recommended work dilution= 1:1000-2000)
Immunogen was synthetic peptide derived from Human Ghrelin precursor. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Supplied as 200 µg of purified and lyophilized antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 200 µg/ml. Please avoid freeze-thaw cycles.
Ghrelin is synthesized as a preprohormone, and then proteolytically processed to yield a 28-amino acid peptide. It is a recently isolated brain-gut peptide that has growth hormone-releasing and appetiteinducing activities. Several recent studies have suggested that ghrelin plays a major role in the pathophysiology of drug-seeking behavior and anxiety. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor peptides that have a signal peptide followed by the ghrelin sequence. At the C-terminus of the ghrelin sequence, processing occurs at an uncommon Pro-Arg recognition site. In rat stomach, two isoforms of mRNA for pro-ghrelin are produced from the gene by an alternative splicing. One mRNA encodes the ghrelin precursor, and another encodes a des-Gln14- ghrelin precursor. O-octanoylation or O-decanoylation is essential for ghrelin activity. The Odecanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26.
The rabbit anti-human Ghrelin antibody detects specifically target derived from related-cell lysates protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
- Zhang J, Ren P, Avsian-Kretchmer O, Luo C, Rauch R, Klein C, Hsueh A (2005). "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake". Science 310 (5750): 9969.
- Garcia J, Garcia-Touza M, Hijazi R, Taffet G, Epner D, Mann D, Smith R, Cunningham G, Marcelli M (2005). "Active ghrelin levels and active to total ghrelin ratio in cancer-induced cachexia". J Clin Endocrinol Metab 90 (5): 29206.
12 months from the date of shipment when stored properly.