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770-729-2992
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Product Specifications
Size:
50 µg
Protein Information
Protein Name & Synonyms:
GLI3
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: , 1/500), Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 0.5mg/ml
Preparation:
Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Human, Amphibia, Chicken, Chimpanzee, Dog, Monkey, Mouse, Quail, Rat
Specificity
This product recognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.
GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.
Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome.GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.
GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.
Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome.GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.
Preservative:
0.09% Sodium Azide (NaN3)
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
