ELISA (recommended work dilution= 1:10e6 (at least)), Western Blotting (recommended work dilution= 2-5 µg/ml)
Immunogen was synthetic peptide derived from Human Hepcidin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction will be purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Hepcidin, also called as LEAP-1 is cysteine-rich peptide containing four disulfide bonds. Its active form, 25-residue peptide was identified in human blood. It was predominantly detected in the liver, and, to a much lower extent, in the heart. Gram-positive Bacillus megaterium, Bacillus subtilis, Micrococcus luteus, Staphylococcus carnosus, and Gram-negative Neisseria cinerea as well as the yeast Saccharomyces cerevisiae dose-dependently exhibited sensitivity upon treatment with synthetic Hepcidin. It is also the central regulator of systemic iron homeostasis. Dysregulation of hepcidin production results in a variety of iron disorders. Hepcidin deficiency is the cause of iron overload in hereditary hemochromatosis, iron-loading anemias, and hepatitis C. Hepcidin excess is associated with anemia of inflammation, chronic kidney disease and iron-refractory iron deficiency anemia.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The antibody detects specifically synthetic immunogen. Cross reactivity with mouse and rat Hepcidin are not tested yet.
- Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation". Blood (2003).
- Park CH, Valore EV, Waring AJ, Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J. Biol. Chem (2001)
12 months from the date of shipment when stored properly.