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| CODE | Antibody Size | Price | ||
|---|---|---|---|---|
|
RB-13-0005-20
|
$52.00 | ||
|
|
RB-13-0005-200
|
$206.00 | ||
|
|
RB-13-0005-500
|
$402.00 | ||
|
|
RB-13-0005-100
|
$155.00 |
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Protein Information
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (recommended work dilution= 1:10^6 (at least)), Western Blotting (recommended work dilution= 2-5 ug/mL)
Immunogen Sequence:
DTHFPICIFCCGCCHRSKCGMCCKT
Preparation:
Immunogen was synthetic peptide derived from Human Hepcidin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction will be purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Reconstitution:
Supplied as lyophilized purified antibody. The antibody can be reconstituted in sterile PBS, be stored at 4°C for 1 month after resuspension. Please avoid freeze-thaw cycles.
Clonality:
Polyclonal
Description
Hepcidin, also called as LEAP-1 is cysteine-rich peptide containing four disulfide bonds. Its active form, 25-residue peptide was identified in human blood. It was predominantly detected in the liver, and, to a much lower extent, in the heart. Gram-positive Bacillus megaterium, Bacillus subtilis, Micrococcus luteus, Staphylococcus carnosus, and Gram-negative Neisseria cinerea as well as the yeast Saccharomyces cerevisiae dose-dependently exhibited sensitivity upon treatment with synthetic Hepcidin. It is also the central regulator of systemic iron homeostasis. Dysregulation of hepcidin production results in a variety of iron disorders. Hepcidin deficiency is the cause of iron overload in hereditary hemochromatosis, iron-loading anemias, and hepatitis C. Hepcidin excess is associated with anemia of inflammation, chronic kidney disease and iron-refractory iron deficiency anemia.
Specificity
The antibody detects specifically synthetic immunogen. Cross reactivity with mouse and rat Hepcidin are not tested yet.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
References
- Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation". Blood (2003).
- Park CH, Valore EV, Waring AJ, Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J. Biol. Chem (2001)
Expiration:
12 months from the date of shipment when stored properly.
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Thinking about using this antibody for western? Try our Auto Western service, which uses capillary immunoblotting to achieve ng to pg sensitivity. Just send us your samples and we will do the rest! Click here to learn more.
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
