Rabbit Anti-Human IFN-gamma [+Biotin]

Rabbit Anti-Human IFN-gamma [+Biotin]

$568.00
DS-PB-01045
+

Antigen Information

Protein Name & Synonyms:
IFN-gamma
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration:
IgG concentration 0.1 mg/ml after reconstitution.
Preparation:
Antisera to human IFN-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human

This product may be used under either reducing or non-reducing western blotting conditions.

Specificity

This product is specific for human IFN-gamma (Interferon-gamma), a 143 amino acid regulatory cytokine and member of the Type II interferon family. IFN-gamma is involved in the regulation of the immune and inflammatory responses and is secreted primarily by mitogen and antigen activated T lymphocytes and natural killer cells (NK).

IFN-gamma is a multifunctional cytokine which signals through the IFN-gamma receptor (IFNGR), found on most immune cells. Properties of IFN-gamma include recruitment of leucocytes to sites of infection, inducement of MHC class I and II antigens, stimulation of anti-microbial and anti-tumour responses of macrophages, Nk cells and neutrophils, and has also been shown to potentiate the antiproliferative effects of IFN-alpha and IFN-beta.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antisera to human IFN-gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human IFN-gamma
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.