Rabbit Anti-Human Insulin-Like Growth Factor II

Rabbit Anti-Human Insulin-Like Growth Factor II (0.1 mg), with HRP-conjugated Secondary Antibody

$571.00
DS-PB-01054
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Antigen Information

Protein Name & Synonyms:
INSULIN-LIKE GROWTH FACTOR II, IGF2, IGF-II, IGFII
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (recommended concentration: 0.5 - 2.0 ug/ml), Functional Assays (recommended concentration: 3.1 - 4.6 ug/ml), Western Blotting (recommended concentration: 0.1 - 0.2 ug/ml)
Concentration:
IgG concentration 1 mg/ml (after reconstitution)
Preparation:
Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.Note: For functional studies do not add azide.
Species Reactivity:
Human

Preparation

Preparation
Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen
Recombinant human IGF-II

Specificity

DS-PB-01054 is specific for human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen. IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research.

Formulation

Product Type
Polyclonal Antibody
Product Form
Purified IgG - lyophilized
Preservative Stabilizers
None Present
Buffer Solution
Phosphate buffered saline
Approx. Protein Concentrations
IgG concentration 1 mg/ml (after reconstitution)

Applications

ELISA
DS-PB-01054 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with DS-PB-02647 as the detection antibody.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months from the date of reconstitution.

Secondary Antibody Applications

Immunoassay (ELISA, Western Blot)
We recommend a dilution of 1:5000 - 1:10000.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.