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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
IL-17F
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml
Preparation:
Antisera to human IL-17F were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
Species Reactivity:
Human
Specificity
This product is specific for human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133 amino acid chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.
IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8. IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis.
This product has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2ug/ml of antibody.
IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8. IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis.
This product has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2ug/ml of antibody.
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide.
N.B. For functional studies do not add sodium azide.
Format Type:
Purified
Preparation
Antisera to human IL-17F were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen:
Recombinant human IL-17F
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
