Protein Name & Synonyms:
IL-31
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human IL-31 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water.. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human
This product is specific for human IL-31 (interleukin-31), a 141 amino acid T-cell derived cytokine and member of the IL-6 cytokine family, which shares homology with oncostatin M, cardiotrophin-1 and LIF (leukaemia inhibitory factor).
IL-31 is preferentially produced by type 2 helper T cells (Th2) and signals through binding to the IL-31 receptor consisting of IL-31 receptor A (IL-31RA/GPL) and oncostatin M receptor B, activators of STAT1, STAT3 and STAT5. IL-31 is an important mediator of T cell mediated immune response and is particularly linked with diseases of the skin, including dermatitis and pruritic atopic skin inflammation, and is also implicated in bronchial inflammation.
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
Antisera to human IL-31 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen:
Recombinant human IL-31.
Preservative:
None present