Rabbit Anti-Human IL-6

Rabbit Anti-Human IL-6

$571.00
DS-PB-01130
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Antigen Information

Protein Name & Synonyms:
IL-6
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunofluorescence, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml after reconstitution
Preparation:
Antisera to human IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Human

This product may be used under reducing or non-reducing conditions.

Specificity

This product is specific for human IL-6 (Interleukin-6), a 184 amino acid multifunctional pro-inflammatory cytokine, secreted by activated T cells, macrophages, endothelial cells, fibroblasts and osteoblasts.

The diverse biological properties of IL-6 include the principle inducer of the acute phase response in the liver, stimulation of B cell differentiation and subsequent immunoglobulin production, promotion of hybridoma growth, regulation of bone metabolism and stimulation of energy mobilisation in muscle and fatty tissue. IL-6 signals through a membrane bound receptor complex consisting of the ligand-binding IL-6Rα chain (CD126) and the signal-transducing component gp130 (CD130), but can also stimulate cells expressing gp130 through a soluble IL-6R.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored udiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Format Type:
Purified

Preparation

Antisera to human IL-6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human IL-6
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.