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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
IL-7
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Preparation:
Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human
Specificity
This product is specific for human IL-7 (Interleukin-7), a 152 amino acid glycoprotein and pleiotropic hematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells.
Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Rα chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.
Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Rα chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.
Storage/Stability
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Format Type:
Purified
Preparation
Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
Immunogen:
Recombinant human IL-7
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
