Rabbit Anti-Human IP-10 / CXCL10

Rabbit Anti-Human IP-10 / CXCL10
CODE:  DS-PB-01174

Antigen Information

Protein Name & Synonyms:
IP-10 / CXCL10
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml after reconstitution
Preparation:
Antisera to human IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human

Specificity

This product is specific for human IP-10 (10-kDa interferon-inducible protein), otherwise known as CXCL10, a 77 amino acid CXC chemokine which, like monokine induced by IFN-gamma (Mig) and IFN-inducible T-cell alpha-chemoattractant (I-TAC), signals through the CXCR3 receptor.

IP-10 acts as a chemoattractant for Th1 lymphocytes and natural killer (NK) cells and is secreted by a variety of cells, including neutrophils, monocytes, keratinocytes and epithelial cells, in response to interferon gamma (IFNgamma) and lipopolysaccharide (LPS). IP-10 is thus associated with several clinical conditions linked with Th1 type immune responses and raised IFNgamma expression, such as psoriasis, sarcoidosis and viral meningitis. In addition, studies have demonstrated the ability of IP-10 to suppress the synergistic action of stimulatory cytokines on hematopoietic progenitor cell proliferation.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human IP-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human IP-10
Preservative:
None present.