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770-729-2992
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Product Specifications
Size:
50 µg
Protein Information
Protein Name & Synonyms:
MCP-1 / CCL2
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration (lot specific):
IgG concentration 0.1 mg/ml after reconstitution.
Preparation:
Antisera to human MCP-1 were raised by repeated immunisations of rabbitts with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human
Specificity
This product is specific for human MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, a 76 amino acid CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2.
The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. Increasing studies have also linked MCP-1 with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.
The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. Increasing studies have also linked MCP-1 with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response.
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Antisera to human MCP-1 were raised by repeated immunisations of rabbitts with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Recombinant human MCP-1.
Preservative:
None present.
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
