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Antigen Information
Protein Name & Synonyms:
MEK1/2 (pSer218/pSer222)
Target Species:
- Human
Antibody Specifications
Applications:
Western Blotting
Preparation:
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, mouse and rat (100% identity with immunogen sequence)
This product recognizes a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.
Specificity
This product is specific for MEK 1 and MEK 2, (also known as MAP2K1 and MAP2K2 respectively), when phosphorylated at serine 218 and 222.
MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.
MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5
Format Type:
Purified
Preparation
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated ser 218 and 222 within human MEK1 and 2.
Preservative:
| 0.09% | Sodium Azide |
| 0.01% | Bovine Serum Albumin |
| 50% | Glycerol |
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
