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Rabbit Anti-Human MEK1/2 (pSer218/pSer222)

Rabbit Anti-Human MEK1/2 (pSer218/pSer222)

$582.00
DS-PB-01311
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Antigen Information

Protein Name & Synonyms:
MEK1/2 (pSer218/pSer222)
Target Species:
  • Human

Antibody Specifications

Applications:
Western Blotting
Preparation:
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, mouse and rat (100% identity with immunogen sequence)

This product recognizes a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.

Specificity

This product is specific for MEK 1 and MEK 2, (also known as MAP2K1 and MAP2K2 respectively), when phosphorylated at serine 218 and 222.

MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5
Format Type:
Purified

Preparation

Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated ser 218 and 222 within human MEK1 and 2.
Preservative:
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.