Protein Name & Synonyms:
MEK1/2 (pSer218/pSer222)
Applications:
Western Blotting
Preparation:
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, mouse and rat (100% identity with immunogen sequence)
This product recognizes a band of approximately 45kD in serum stimulated mouse NIH 3T3 cell lysates.
This product is specific for MEK 1 and MEK 2, (also known as MAP2K1 and MAP2K2 respectively), when phosphorylated at serine 218 and 222.
MEK1 and 2 are related dual specificity protein kinases from the MAP kinase family. These proteins are activated by phosphoryation at serine 218 and 222, and play a critical role in mitogen growth factor signal transduction.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5
Antisera to phosphorylated MEK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence which includes phosphorylated ser 218 and 222 within human MEK1 and 2.
Preservative:
0.09% | Sodium Azide |
0.01% | Bovine Serum Albumin |
50% | Glycerol |
Expiration:
12 months from the date of shipment when stored properly.