ELISA (recommended work dilution= 1 : 16,000)
Immunogen was synthetic peptide derived from human MSH-alpha. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
The melanocyte-stimulating hormones (MSH) are a class of peptide hormones that are produced by cells in the intermediate lobe of the pituitary gland. MSH belongs to a group called the melanocortins. This group includes ACTH, alpha-melanocyte-stimulating hormone (alpha-MSH), beta-melanocyte-stimulating hormone (beta-MSH) and gamma-melanocyte-stimulating hormone (?-MSH); these peptides are all cleavage products of a large precursor peptide called pro-opiomelanocortin (POMC). alpha-MSH is the most important melanocortin for pigmentation.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The antibody can specifically bind to human MSH-alpha. No cross reactivity was observed with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse or rat MSH-alpha was not tested.
- Hadley ME (Oct 2005). "Discovery that a melanocortin regulates sexual functions in male and female humans". Peptides 26 (10): 16879. Millington GW (May 2006). "Proopiomelanocortin (POMC): the cutaneous roles of its melanocortin products and receptors". Clin. Exp. Dermatol. 31 (3): 40712
- Millington GW (2007). "The role of proopiomelanocortin (POMC) neurones in feeding behaviour". Nutr Metab (Lond) 4: 18.
12 months from the date of shipment when stored properly.