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Antigen Information
Protein Name & Synonyms:
Myosin Regulatory Light Chain
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 1/500, 1/2000), Immunohistology - Paraffin, Western Blotting
Concentration:
0.5 mg/ml
Host Species:
Rabbit
Species Reactivity:
Human, Birds, Mammals, Mouse, Rat
Specificity
This antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.
Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.
Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Immunogen:
A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.
Preservative:
0.01% Sodium Azide (NaN3)
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
