Rabbit Anti-Human NFkB p65 (pSer536)

Rabbit Anti-Human NFkB p65 (pSer536)

DS-PB-02714
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Antigen Information

Protein Name & Synonyms:
NFkB p65 (pSer536)
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 1/100, 1/1000), Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Human, Mouse

This product detects a band of approximately 65kDa in the appropriate cell lysate.

Specificity

This product detects the p65 (RelA) subunit of nuclear factor kappa-B (NFkB) when phosphorylated at serine 536. NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits. Research has led to the discovery of other subunits, including p52 (NFkB2), cRel, and RelB.

NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.

Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to NFkB p65 (pSer536) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:
Synthetic peptide corresponding to residues surrounding serine 536 of human NFkB p65 protein.
Preservative:
0.09% Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.