Rabbit Anti-Human PAK2 (C-term)

Rabbit Anti-Human PAK2 (C-term)

DS-PB-01536
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Antigen Information

Protein Name & Synonyms:
PAK2
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human PAK2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Expected cross reactivity from sequence similarity: mouse and rat

This product detects a band of approximately 58kDa in Jurkat cell lysates.

Specificity

This product recognizes p21-activated kinase-2 (PAK2), a ubiquitously expressed, 64kDa, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen. p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain. Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling. Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding.

PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals. Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response. This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus. PAK2 may also play a role in X-linked mental retardation.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human PAK2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
14 amino acid peptide sequence near the carboxy terminus of human PAK2.
Preservative:
0.02%Sodium Azide

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.