Rabbit anti-POMC [+Biotin]

Rabbit anti-POMC Biotinylated Antibody, 50 ug

$220.00  
RB-08-0013B-50
+

Protein Information

Accession Number:
  • NM_001035256
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
Conjugation/Tag:
Biotin
Host Species:
Rabbit
Preparation:
Immunogen was synthetic peptide derived from Human POMC. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Reconstitution:
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Clonality:
Polyclonal
Isotype:
IgG

Description

The proopiomelanocortin (POMC) gene is expressed in the pituitary and arcuateneurons of the hypothalamus. POMC arcuate neurons play a central role in the control of energy homeostasis. The POMC precursor hormone undergoes enzymatic cleavage to yield active ligands for melanocortin receptors. However, processing is incomplete in some tissues,leading to POMC precursor release from cells. POMC gene encodes for several biologically active peptides, including ACTH, beta-lipotropin (beta- LPH), alpha-, beta-, and ?-melanocyte stimulating hormone (alpha-, beta-, ?-MSH) and beta-endorphin, which affect pigmentation; the immune, central, and peripheral nervous systems; adrenocortical function; and energy regulation . Recently, a QTL for leptin serum levels and fat mass was localized to HSA2q21, making POMC a strong positional candidate gene for human obesity studies. Additional studies have shown that mice lacking POMC-derived peptides are obese and have affected adrenal development and pigmentation, much like humans with POMC deficiency. The CNS POMC system has other functions, including regulation of sexual behaviour, lactation, the reproductive cycle and possibly central cardiovascular control.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Specificity

The rabbit anti-human POMC antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.

References

  • Yaswen, L. et.al. Obesity in the mouse model of pro-opiomelanocortin deficiency responds to peripheral melanocortin.Nature Med. 5: 1066-1070, 1999.
  • Santoro, N. et.al. An insertional polymorphism of the proopiomelanocortin gene is associated with fasting insulin levels in childhood obesity. J. Clin. Endocr. Metab. 89: 4846-4849, 2004.
Expiration:
12 months from the date of shipment when stored properly.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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