Rabbit Anti-Human TGF alpha

Rabbit Anti-Human TGF alpha

$571.00
DS-PB-01936
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Antigen Information

Protein Name & Synonyms:
TGF alpha
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Preparation:
Antisera to human TGF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.
Reconstitution:
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human
Immunohistology:
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.

Specificity

This product is specific for human TGF-alpha (transforming growth factor alpha), a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).

TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human TGF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.

Immunogen:
E.cloli derived recombinant human TGF-alpha
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.