Rabbit anti-Visfatin [+Biotin]

Rabbit anti-Visfatin Biotinylated Antibody, 50 ug


Protein Information

Accession Number:
  • NM_182790
Target Species:
  • Human

Antibody Specifications

ELISA (recommended work dilution= 1:10e6(at least detecting 10 µg/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 µg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
Host Species:
Immunogen was synthetic peptide derived from Human Visfatin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.


Visfatin also designated Pre-B cell-enhancing factor (PBEF) or Nicotinamide hosphoribosyltransferase (Nampt) , belongs to the NAPRTase family of proteins. Visfatin is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor, albeit in a different place than insulin. Furthermore, Visfatin may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. More recently, several groups have reported the crystal structure of Nampt/PBEF/visfatin and they all show that this protein is a dimeric type II phosphoribosyltransferase enzyme involved in NAD biosynthesis pathway. Visfatin is a cytoplasmic protein with a molecular weight of 52 kDa composed of 473-amino acid. It has a hydrophobic N terminus, 2 N-glycosylation sites, several putative phosphorylation sites, and 6 cysteines. Visfatin is expressed primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.


The rabbit anti-human Visfatin antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.

Related Products

Visfatin ELISA Kit(cat#ELH-Visfatin-001); Visfatin Immunodetection Kit (Western-blot-based, cat#RB-08-0003K) ; Visfatin Immunoassay Kit (EIA, cat# EIA-Vis-1); Obesity antibody arrays(cat#AAH-OBS-1-1)


  • Fukuhara A Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science. 2005 Jan 21;307(5708):426-30. Epub 2004 Dec 16. PMID: 15604363
  • Hug C, Lodish HF. Medicine. Visfatin: a new adipokine. Science. 2005 Jan 21;307(5708):366-7. PMID: 15604359
  • A. Fukuhara, et al., "Visfatin: a protein secreted by visceral fat that mimics the effects of insulin," Science, published online Dec. 16, 2004.
12 months from the date of shipment when stored properly.

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