Rabbit Anti-Human Visfatin

Rabbit Anti-Human Visfatin Antibody

$52.00  
RB-08-0003-20
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Protein Information

Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100pg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
Immunogen Sequence:
CVTKSYSFDEIRKNAQLNIELEAAHH
Preparation:
Immunogen was synthetic peptide derived from Human Visfatin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Reconstitution:
Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 µg/ml. Please avoid freeze-thaw cycles.

Description

Visfatin also designated Pre-B cell-enhancing factor (PBEF) or Nicotinamide hosphoribosyltransferase (Nampt) , belongs to the NAPRTase family of proteins. Visfatin is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor, albeit in a different place than insulin. Furthermore, Visfatin may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. More recently, several groups have reported the crystal structure of Nampt/PBEF/visfatin and they all show that this protein is a dimeric type II phosphoribosyltransferase enzyme involved in NAD biosynthesis pathway. Visfatin is a cytoplasmic protein with a molecular weight of 52 kDa composed of 473-amino acid. It has a hydrophobic N terminus, 2 N-glycosylation sites, several putative phosphorylation sites, and 6 cysteines. Visfatin is expressed primarily in bone marrow, muscle and liver tissue but can also be detected in placenta, lung, kidney and heart tissue.

Specificity

The rabbit anti-human Visfatin antibody detects specifically target derived from human recombinant protein as well as cell lysates of MD231 and Hela at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.

Storage

Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.

Related Products

Visfatin ELISA Kit(cat#ELH-Visfatin-001); Visfatin Immunodetection Kit (Western-blot-based, cat#RB-08-0003K) ; Visfatin Immunoassay Kit (EIA, cat# EIA-Vis-1); Obesity antibody arrays(cat#AAH-OBS-1-1)

References

  • Fukuhara A Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science. 2005 Jan 21;307(5708):426-30. Epub 2004 Dec 16. PMID: 15604363
  • Hug C, Lodish HF. Medicine. Visfatin: a new adipokine. Science. 2005 Jan 21;307(5708):366-7. PMID: 15604359
  • A. Fukuhara, et al., "Visfatin: a protein secreted by visceral fat that mimics the effects of insulin," Science, published online Dec. 16, 2004.
Expiration:
12 months from the date of shipment when stored properly.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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