Rabbit Anti-Human WNT1

Rabbit Anti-Human WNT1

DS-PB-02787
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Antigen Information

Protein Name & Synonyms:
WNT1
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water.. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human

Specificity

This product specifically recognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.

Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
Format Type:
Purified

Preparation

Antisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:
Recombinant human Wnt-1
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.