Protein Name & Synonyms:
LPCAT2, AGPAT11, AYTL1, LysoPAFAT, lysophosphatidylcholine acyltransferase 2
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 160-544 of human LPCAT2 (NP_060309.2).
Western blot analysis of extracts of various cell lines, using LPCAT2 antibody (144-04805) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.