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Antigen Information

Protein Name & Synonyms:

CINC-1 / GRO alpha / CXCL1

Target Species:

Mouse

Antibody Specifications

Applications:

ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Western Blotting

Concentration:

IgG concentration 50ug/ml after reconstitution.

Preparation:

Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Reconstitution:

Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Species Reactivity:

Mouse

This product may be used under either reducing or non-reducing western blotting conditions.

Specificity

This product recognizes mouse CXCL1 also known as GRO1 and KC, a 7.8kDa chemokine involved in neutrophil chemotaxis and activation, and expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:

Purified IgG conjugated to Biotin - lyophilized

Formulation:

Phosphate buffered saline

Reconstitution:

Reconstitute with 1.0ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antisera to mouse CXCL1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:

Recombinant mouse CXCL1

Preservative: