Protein Name & Synonyms:
IFN-gamma
Applications:
ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml after reconstitution
Preparation:
Antisera to mouse interferon gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Mouse
This product is specific for mouse interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antisera to mouse interferon gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant mouse interferon gamma
Preservative:
None Present
Expiration:
12 months from the date of shipment when stored properly.