Protein Name & Synonyms:
IL-10
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml after reconstitution.
Preparation:
Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Mouse
This product is specific for mouse Interleukin-10 (IL-10), an immunosuppressive cytokine originally isolated from T helper type 2 cells (Th2), which is also secreted by B lymphocytes, macrophages, monocytes and keratinocytes.
IL-10 inhibits the synthesis of several cytokines including Tumour Necrosis Factor, Interferon-gamma and Interleukin-2 and -3, and has a wide range of biological properties, including inhibition of the macrophage and Th cell-mediated cytokine synthesis suppression of the delayed hypersensitivity response. Reports suggest that IL-10 may initially be involved in the polarization of Th2 responses.
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant mouse IL-10
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
