ELISA (recommended work dilution= 1:10,000)
The immunogen was a synthetic peptide containing two discontinuous domains derived from mouse IL-17F. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation and followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Interleukin-17 (IL-17A) is a cytokine secreted exclusively by activated T-cells. The human IL-17A gene product is a protein of 150 amino acids with a molecular weight of 15 kDa, and is secreted as a disulfide linked homodimer of 3035 kDa glycoprotein. Five related cytokines were identified, through database searches and degenerative RT-PCR, that share 2050% homology to IL-17. IL-17 has been designated IL-17A to indicate that it is the founding member of the IL-17 cytokine family. The shared features of the IL-17 cytokine family include conserved cysteines which, in IL-17F, have been shown to exhibit the features of a classic cystine knot structural motif found in bone morphogenetic proteins (BMPs), transforming growth factor beta (TGF-_), nerve growth factor (NGF) and platelet-derived growth factor BB (PDGF-BB). IL-17F, like IL-17A, is produced primarily in activated T-cells. In contrast, IL-17B, IL-17C, IL-17D, and IL-17E are expressed in a wide assortment of tissues. Their functions partially overlap those of IL-17A, although they have not been as thoroughly investigated.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.