Protein Name & Synonyms:
IL-2
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration (lot specific):
IgG concentration 0.1mg/ml after reconstitution
Preparation:
Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Mouse
This product is specific for mouse Interleukin-2 (IL-2), a potent lymphoid cell growth factor which primarily acts on T lymphocytes, in response to antigenic or mitogenic stimulation.
IL-2 stimulates the growth/differentiation of monocytes, macrophages, B lymphocytes, NK cells, and lymphokine activated killer cells which are used in experimental cancer therapy.
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant mouse IL-2
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.