Rabbit Anti-Mouse IL-4

Rabbit Anti-Mouse IL-4

DS-PB-01116
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Antigen Information

Protein Name & Synonyms:
IL-4
Target Species:
  • Mouse

Antibody Specifications

Applications:
ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml after reconstitution.
Preparation:
Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add azide.
Species Reactivity:
Mouse

Specificity

This product specifically recognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and hematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:
Recombinant mouse IL-4
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.