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Rabbit Anti-Mouse MDM2

Rabbit Anti-Mouse MDM2

$359.00  
DS-PB-02829
+ −

Antigen Information

Protein Name & Synonyms:
MDM2
Target Species:
  • Mouse

Antibody Specifications

Applications:
ELISA (dilution: 1/500, 1/2000), Western Blotting
Concentration:
IgG concentration 0.5mg/ml
Preparation:
Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Mouse

This product detects a band of approximately 90kDa in mouse MEF cell lysates.

Specificity

This product detects murine Mdm2, a nuclear phosphoprotein that controls intracellular levels of the tumour suppressor protein p53 by ubiquitination and subsequent proteasomal degradation. Mdm2 also interacts with the retinoblastoma (RB) protein and E2F-1, to promote G1-S phase transition in the cell cycle.

Mdm2 activity is controlled by self-ubiquitination; however activation of the PI3K/PKB pathway leads to phosphorylation of Mdm2 at Ser185, which stabilises the protein.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to Mdm2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Synthetic peptide corresponding to aa 177-195 of mouse Mdm2.
Preservative:
0.09% Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.