Rabbit Anti-Mouse MIP-2 / CXCL2

Rabbit Anti-Mouse MIP-2 / CXCL2

DS-PB-01339
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Antigen Information

Protein Name & Synonyms:
MIP-2 / CXCL2
Target Species:
  • Mouse

Antibody Specifications

Applications:
Immunohistology - Frozen, ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 0.1 mg/ml after reconstitution.
Preparation:
Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Mouse

This product may be used under either reducing or non-reducing conditions.

Immunohistology:
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.

Specificity

This product is specific for mouse Macrophage Inflammatory Protein-2 (MIP-2), also known as CXCL2.

MIP-2 is expressed by macrophages and epidermal langerhans cells and is chemotactic for neutrophils.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Format Type:
Purified

Preparation

Antisera to mouse MIP-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:
Recombinant mouse MIP-2
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.