Rabbit Anti-Mouse NGF beta

Rabbit Anti-Mouse NGF beta

DS-PB-01453
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Antigen Information

Protein Name & Synonyms:
NGF beta
Target Species:
  • Mouse

Antibody Specifications

Applications:
ELISA (dilution: 0.1ug/ml, 1.0ug/ml), Functional Assays, Western Blotting
Concentration:
IgG concentration1.0 mg/ml after reconstitution
Preparation:
Antiserum to NGF 2.5S was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
Reconstitution:
Reconstitute with 1.0ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Species Reactivity:
Mouse, Human

Specificity

This product recognizes both the 2.5S and 7S forms of mouse nerve growth factor beta, a secreted peptide involved in the development and maintenance of the sympathetic and sensory nervous systems.

In a standard bioassay, this antibody neutralises the activity of 3.0 ng/ml NGF 2.5S when used at a concentration of around 0.5 ug/ml.

Storage/Stability

Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Format Type:
Purified

Preparation

Antiserum to NGF 2.5S was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.

Immunogen:
Affinity purified mouse NGF 2.5S
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.