Protein Name & Synonyms:
TNRC6A, CAGH26, GW1, GW182, TNRC6, trinucleotide repeat containing 6A
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.
WB 1:500 - 1:1000
IF 1:50 - 1:100
A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
Western blot analysis of extracts of various cell lines, using TNRC6A antibody (144-06115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using TNRC6A antibody (144-06115) at dilution of 1:100. Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.