Rabbit Anti-Mouse TNF-alpha

Rabbit Anti-Mouse TNF-alpha

DS-PB-01976
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Antigen Information

Protein Name & Synonyms:
TNF-alpha / TNF
Target Species:
  • Mouse

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to mouse TNF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. The addition of 0.09% sodium azide is recommended for long term storage. N.B. For functional studies do not add azide.
Species Reactivity:
Mouse

Specificity

This product is specific for mouse Tumour Necrosis Factor alpha (TNF❏❏alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.

TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Storage/Stability

Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
The addition of 0.09% sodium azide is recommended for long term storage.
N.B. For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to mouse TNF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant mouse TNF-alpha
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.