Protein Name & Synonyms:
MTM1, CNM, MTMX, XLMTM, myotubularin
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a dual-specificity phosphatase that acts on both phosphotyrosine and phosphoserine. It is required for muscle cell differentiation and mutations in this gene have been identified as being responsible for X-linked myotubular myopathy.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 484-603 of human MTM1 (NP_000243.1).
Western blot analysis of extracts of various cell lines, using MTM1 antibody (144-00255) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.