Western blot analysis of extracts of various cell lines, using NAA50 antibody (144-07387) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using NAA50 Antibody (144-07387) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestine using NAA50 Antibody (144-07387) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using NAA50 antibody (144-07387). Blue: DAPI for nuclear staining.
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