Protein Name & Synonyms:
NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1).
Western blot analysis of extracts of various cell lines, using NDC80 antibody (144-05411) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.