Rabbit anti-NDC80 Antibody

NDC80 Polyclonal Antibody

$200.00  
144-05411-50
+

Antigen Information

Accession Number:
  • O14777
Gene ID:
  • 10403
Gene Symbols:
  • NDC80
Protein Name & Synonyms:
NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80, kinetochore protein NDC80 homolog
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
73kDa
Observed MW
80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NDC80 antibody (144-05411) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - NDC80 antibody (144-05411)

No posts found

Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.