Protein Name & Synonyms:
NR2C1, TR2, nuclear receptor subfamily 2 group C member 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2).
Western blot analysis of extracts of various cell lines, using NR2C1 antibody (144-06675) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using NR2C1 antibody (144-06675).