Protein Name & Synonyms:
PAH, PH, PKU, PKU1, phenylalanine hydroxylase
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Human, Mouse, Rat
PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PAH (NP_000268.1).
Western blot analysis of extracts of various cell lines, using PAH antibody (144-01559) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.