Protein Name & Synonyms:
PARD6A, PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40, partitioning defective 6 homolog alpha
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cell membrane protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. The protein also has a role in the epithelial-to-mesenchymal transition (EMT) that characterizes the invasive phenotype associated with metastatic carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
WB 1:500 - 1:2000
A synthetic peptide of human PARD6A
Western blot analysis of extracts of various cell lines, using PARD6A antibody (144-03064) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.