Rabbit anti-PEX14 Antibody

PEX14 Polyclonal Antibody

$200.00  
144-07336-50
+

Antigen Information

Accession Number:
  • O75381
Gene ID:
  • 5195
Gene Symbols:
  • PEX14
Protein Name & Synonyms:
PEX14, NAPP2, PBD13A, Pex14p, dJ734G22.2, peroxisomal biogenesis factor 14
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IHC,IF
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
36kDa/41kDa
Observed MW
41kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 118-377 of human PEX14 (NP_004556.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse liver, using PEX14 antibody (144-07336) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - PEX14 antibody (144-07336)

Immunohistochemistry of paraffin-embedded human prostate cancer using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry - PEX14 antibody (144-07336)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry - PEX14 antibody (144-07336)

Immunohistochemistry of paraffin-embedded human liver injury using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry - PEX14 antibody (144-07336)

Immunohistochemistry of paraffin-embedded human lung cancer using PEX14 antibody (144-07336) at dilution of 1:100 (40x lens).
Immunohistochemistry - PEX14 antibody (144-07336)

Immunofluorescence analysis of A549 cells using PEX14 antibody (144-07336). Blue: DAPI for nuclear staining.
Immunofluorescence - PEX14 antibody (144-07336)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.