The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:200
Format
Calculated MW
28kDa/36kDa/40kDa
Observed MW
62kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T232 of human FOS
Modification
Phospho T232
Storage
-20°C
Images
Western blot analysis of extracts of HepG2 cells, using Phospho-FOS-T232 antibody (144-50038) at 1:1000 dilution. HepG2 cells were treated by 10% FBS for after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunofluorescence analysis of MCF-7 cells using Phospho-FOS-T232 antibody (144-50038). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
