Rabbit anti-Phospho-PRKACA-T197 Antibody

Phospho-PRKACA-T197 Polyclonal Antibody

$270.00
144-50557-50
+

Antigen Information

Accession Number:
  • P17612
Gene ID:
  • 5566
Gene Symbols:
  • PRKACA
Protein Name & Synonyms:
PRKACA, PKACA, PPNAD4, cAMP-dependent protein kinase catalytic subunit alpha
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse, Rat
Isotype:
IgG

Background

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
39kDa/40kDa
Observed MW
40kDa

Immunogen

A phospho specific peptide corresponding to residues surrounding T197 of human PRKACA

Modification

Phospho T197

Storage

-20°C

Images

Western blot analysis of extracts of 293T cells,using Phospho-PRKACA-T197 antibody (144-50557) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot - Phospho-PRKACA-T197 pAb (144-50557)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.