Rabbit anti-PLA2G1B Antibody

PLA2G1B Polyclonal Antibody
CODE:  144-61418-50

Antigen Information

Accession Number:
  • P04054
Gene ID:
  • 5319
Gene Symbols:
  • PLA2G1B
Protein Name & Synonyms:
PLA2G1B, PLA2, PLA2A, PPLA2, phospholipase A2
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
16kDa
Observed MW
16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-148 of human PLA2G1B (NP_000919.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PLA2G1B antibody (144-61418) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - PLA2G1B antibody (144-61418)