Protein Name & Synonyms:
PLAU, ATF, BDPLT5, QPD, UPA, URK, u-PA, plasminogen activator, urokinase
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 20-180 of human PLAU (NP_002649.1).
Western blot analysis of extracts of various cell lines, using PLAU antibody (144-02181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.