Rabbit anti-PLCB1 Antibody

PLCB1 Polyclonal Antibody

$320.00  
144-01971-100
+

Antigen Information

Accession Number:
  • Q9NQ66
Gene ID:
  • 23236
Gene Symbols:
  • PLCB1
Protein Name & Synonyms:
PLCB1, EIEE12, PI-PLC, PLC-154, PLC-I, PLC-beta-1, PLC154, PLCB1A, PLCB1B, phospholipase C beta 1
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IHC,IF
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Isotype:
IgG

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
133kDa/138kDa
Observed MW
145kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (144-01971) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PLCB1 antibody (144-01971)

Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLCB1 antibody (144-01971)

Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLCB1 antibody (144-01971)

Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (144-01971) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLCB1 antibody (144-01971)

Immunofluorescence analysis of U2OS cells using PLCB1 antibody (144-01971). Blue: DAPI for nuclear staining.
Immunofluorescence - PLCB1 antibody (144-01971)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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