Protein Name & Synonyms:
PNP, NP, PRO1837, PUNP, purine nucleoside phosphorylase
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
WB 1:500 - 1:2000
IP 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2).
Western blot analysis of extracts of various cell lines, using PNP antibody (144-05778) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.