Rabbit anti-PNP Antibody

PNP Polyclonal Antibody

$200.00  
144-05778-50
+

Antigen Information

Accession Number:
  • P00491
Gene ID:
  • 4860
Gene Symbols:
  • PNP
Protein Name & Synonyms:
PNP, NP, PRO1837, PUNP, purine nucleoside phosphorylase
Target Species:
  • Human

Antibody Specifications

Applications:
WB, IP
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:100

Format

Calculated MW
32kDa
Observed MW
30kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PNP antibody (144-05778) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - PNP Polyclonal Antibody (144-05778)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.