POLR1D, AC19, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2, RNA polymerase I subunit D
Accession Number
Q9Y2S0
Gene Symbols
POLR1D
Gene Id
51082
Recommended Applications
Western Blotting,IF
Purity
Affinity purification
Background
The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:100
Format
Calculated MW
14kDa/15kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR1D antibody (144-08021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Immunofluorescence analysis of A549 cells using POLR1D antibody (144-08021). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
