Rabbit anti-POLR3K Antibody

POLR3K Polyclonal Antibody

$200.00
144-06467-50
+

Antigen Information

Accession Number:
  • Q9Y2Y1
Gene ID:
  • 51728
Gene Symbols:
  • POLR3K
Protein Name & Synonyms:
POLR3K, C11, C11-RNP3, My010, RPC10, RPC11, RPC12.5, RNA polymerase III subunit K
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
12kDa
Observed MW
12kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using POLR3K antibody (144-06467) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - POLR3K antibody (144-06467)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.